Notch-1 (hN1, LIN12, Lin-12, MIS6, Neurogenic Locus Notch Homolog Protein 1, NOTC1, Notch (Drosophila) Homolog 1 (Translocation Associated), Notch 1 Extracellular Truncation, Notch 1 Intracellular Domain, Notch Homolog 1, Notch Homolog 1 Translocation Associated (Drosophila), Translocation Associated (Drosophila), Translocation Associated Notch Homolog, Translocation Associated Notch Protein TAN1)

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£889.00

SKU:: N5375-03J
Reinheit:: Purified by immunoaffinity chromatography
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to Ala19-Gln526 from mouse Notch-1 expressed in CHO cells.
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Weitere Details:: Notch-1 (so named for “notches” in fly wings; also TAN-1) is a 300kD, 2538aa member of the notch family of type I TM glycoproteins. It is associated with gene activation in both embryo and adult. Applications: Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using bEnd.3 mouse endothelioma cell line Immunohistochemistry: 5-15ug/ml using immersion fixed frozen sections of mouse neural tube (E13.5) Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.