N2851-ML490
Nkx 5.1 (NK-2 Family Homeodomain Transcription Factor) (MaxLight 490)
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£1,070.00
- SKU:
- N2851-ML490
- Reinheit:
- Purified by affinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa311-328 of human Nkx5.1
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
- Weitere Details:
- MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Applications: Suitable for use in FLISA, Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Immunohistochemistry Protocol: 1. Dissect tissues and freeze on dry ice 2. Cut on a cryostat -10um slices 3. Dry slides at RT for 30-90 minutes 4. Fix with cold Acetone/Methanol (50/50) 2 minutes - then either process further or store at -20°C . Alternative is fixation with 4% PFA and treatment with a trypsin solution. (0.05% trypsin, incubation 10 minutes at 37°C, followed by 3 washes with PBS 5. Let air dry 6. PBS 5 minutes 7. Incubate in 50mM ammonium chloride 30 minutes 8. PBS 5 minutes 9. Blocking serum 30-45 minutes 10. N2851 90 minutes - dilution 1:200 - 1:1000 11. Wash 3 times for 5 minutes 12. Secondary fluorescent conjug. antibody 30 minutes 13. Wash 3 times for 5 minutes and mount Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice