Nkx 5.1 (NK-2 Family Homeodomain Transcription Factor) (FITC)

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£1,070.00

SKU:: N2851-FITC
Reinheit:: Purified by affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa311-328 of human Nkx5.1
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Weitere Details:: Applications: Suitable for use in FLISA, Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Immunohistochemistry Protocol: 1. Dissect tissues and freeze on dry ice 2. Cut on a cryostat -10um slices 3. Dry slides at RT for 30-90 minutes 4. Fix with cold Acetone/Methanol (50/50) 2 minutes - then either process further or store at -20°C . Alternative is fixation with 4% PFA and treatment with a trypsin solution. (0.05% trypsin, incubation 10 minutes at 37°C, followed by 3 washes with PBS 5. Let air dry 6. PBS 5 minutes 7. Incubate in 50mM ammonium chloride 30 minutes 8. PBS 5 minutes 9. Blocking serum 30-45 minutes 10. N2851 90 minutes - dilution 1:200 - 1:1000 11. Wash 3 times for 5 minutes 12. Secondary fluorescent conjug. antibody 30 minutes 13. Wash 3 times for 5 minutes and mount Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

Versandbedingungen:: Blue Ice