N2851-75H-HRP

NKG2A/C/E (Killer Cell Lectin-like Receptor Subfamily C, Member 1/2/3, Natural Killer Cell Protein Group 2-A1, Natural Killer Cell Protein Group 2-C1, Natural Killer Cell Receptor NKG2A, Natural Killer Cell Receptor NKG2C, Natural Killer Cell Group 2E Cell Receptor NKG2E) (HRP)

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£1,029.00

SKU:: N2851-75H-HRP
Reinheit:: Purified
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rat
Immunogen:: Stable Chinese hamster ovary (CHO) cells expressing the B6 alleles of CD94 and NKG2A.
Klon:: 20D5
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Weitere Details:: Killer cell lectin-like receptor subfamily C, member 1/2/3 (UniProt: Q9Z202/ Q9WTJ9/Q9QXN7; also known as Natural killer cell protein group 2-A1/ Natural killer cell protein group 2-C1, Natural killer cell receptor NKG2A/Natural killer cell receptor NKG2C/ Natural killer cell group 2E cell receptor NKG2E) are encoded by the Klrc1/2/3 genes (Gene IDs: 16641/ 16642/58179) in murine species. The NKG2 proteins are members of the lectin-like receptor family that heterodimerize with CD94 on the surface of NK and NKT cells. They are also shown to be expressed on activated CD8+ T cells. They are integral component of membrane and are present on the external side of plasma membrane. Within their extracellular carbohydrate recognition domains, NKG2A, NKG2C and NKG2E share extensive homology; however, NKG2C and NKG2E receptors differ significantly from NKG2A in their cytoplasmic domains. NKG2/CD94 heterodimeric complexes are shown to recognize Qa-1 that is described as a nonclassical MHC class I antigen. These heterodimers are reported to transduce signals following ligand binding. NKG2A is shown to be inhibitory in nature, while NKG2C and NKG2E transduce stimulatory signals. Comparatively, NKG2A is more abundant than NKG2C and NKG2E in NK cells. Murine NKG2A contains two intracytoplasmic sequences that closely resemble the Immunoreceptor Tyrosine- based Inhibitory Motif (ITIM). N2851-75H can be used for the identification of NK cells expressing functional CD94/NKG2 receptors and is suitable for blocking NKG2A signals in murine tumor models. (Ref.: Vance, RE., et al. (1999). J. Exp. Med. 190(12); 1801-1812). Applications: Suitable for use in Inhibition Assays. Other applications not tested. Recommended Dilution: Optimal dilution determined by the researcher Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

Versandbedingungen:: Blue Ice