N2750-10A

NIS (Sodium Iodide Symporter, Na(+)/I(-) Symporter, Sodium/iodide Cotransporter, Na(+)/I(-) Cotransporter, Solute Carrier Family 5 Member 5, SLC5A5)

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£626.00

SKU:: N2750-10A
Anwendung:: WB
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Mannose binding protein hNIS fusion (aa468-643).
Klon:: 14F
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.
Weitere Details:: The sodium iodide symporter (NIS) is an ion pump that actively transports iodide across the basolateral membrane into thyroid epithelial cells (1, 2). This is mportant step in the process of iodide organificaton and the formation of triiodothyronine and thyroxine (3). Applications: Suitable for use Western Blot. Other applications not tested. Recommended Dilution: Western Blot (ECL): 1:1000 detects hNIS in 20ug of transfected COS-7 cell membrane lysate by using Goat anti-mouse IgG:HRP. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.