N2562-01
Nicotinic Acetylcholine Receptor (nAChR, CHRND, ACHRD, nAChR delta)
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£767.00
- SKU:
- N2562-01
- Reinheit:
- Ascites.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified Torpedo californica AChR (acetylcholine receptor).
- Klon:
- 6D608 (88B)
- Format:
- Supplied as a liquid, 0.05% sodium azide.
- Weitere Details:
- Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine. The nAChRs fall into two subclasses, the muscle-type and the neuronal-type. The muscle-type receptor is a pentameric glycoprotein of five membrane spanning subunits, (2 alpha 1’s, beta 1, gamma or epsilon and delta) which form a ligand gated ion channel. The neuronal nAChR is also a pentamer, but unlike the muscle-type, is made up of only two general types of subunits, alpha and beta of which multiple subtypes have been described (alpha 2 to alpha 8 and beta 2 to beta 4). Applications: Suitable for use in Immunohistochemistry, Immunoprecipitation, and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Frozen): 1:1000 Immunoprecipitation: 1:10 Western Blot: 1:5000 Bands at ~60kD (gamma), ~65kD (delta) and ~130kD (dimer of delta) under non-reducing conditions Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice