N2302-03-HRP
NFkB, p65 (NF-kB, Nuclear Factor kappa B, RelA) (HRP)
Größe
£1,049.00
- SKU:
- N2302-03-HRP
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Peptide corresponding to human p65 coupled to BSA.
- Klon:
- 2Q2254 (12H11)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
- Weitere Details:
- The nuclear factor kB (NFkB) is a sequence-specific DNA-binding protein (2). It is a pleiotropic transcription factor which is involved in the expression of a variety of cellular and viral genes (3). NFkB consists of two subunits which are named according to their molecular weight, p50 and p65 (4). The subunits are stabilized by a so-called inhibitory chain IkB (5). In quiescent cells, NFkB resides in the cytosol in an inactive form which can be activated in vivo by treatment of cells with cytokines or protein kinase activators (6). In vitro, it is possible to generate the active form of NFkB by treatment with sodium deoxycholate, formadine or electrophoretic size fractionation. The active form of NFkB is a heterotetrameric protein, consisting of the two p50 and two p65 subunits (7). After activation NFkB translocates from the cytosol to the nucleus of the cell, binds to specific DNA sequences and initiates transcription. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot, Gel Supershift, Electrophoretic mobility supershift assays (EMSA), Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (paraffin): APAAP Requires HIER using sodium citrate buffer pH6.0 prior to staining. Immunohistochemistry (frozen): APAAP Acetone or methanol fixed cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice