N2302-01B
NFkB, p65, phosphorylated (Ser536) (NF Kappa B, NF-kB, Nuclear Factor kappa B)
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£672.00
- SKU:
- N2302-01B
- Reinheit:
- Purified
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-kB p65. Species sequence homology: canine.
- Klon:
- 4i11(93H1)
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
- Weitere Details:
- Transcription factors of the nuclear factor kB (NF-kB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). In mammals the family, which is comprised of five proteins, Rel-A, c-Rel, Rel-B, NF-kB1 (p105/p50), and NF-kB2 (p100/p52). p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. The p50 and p52 products form dimeric complexes with Rel proteins, which are then able to bind DNA and regulate transcription. In unstimulated cells, NF-kB is sequestered in the cytoplasm by its inhibitory proteins, the IkBs (3–5). NF-kB-activating agents can induce the phosphorylation of IkBs, which targets them for rapid degradation through a ubiquitin-proteasome pathway, releasing NF-kB to enter the nucleus and regulate gene expression (6–8). Processing of NF-kB2 is regulated by IKK1 (IKK-a), which triggers the phosphorylation and processing to p52, which can then undergo nuclear translocation (9-11). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (IC): 1:800 -1:3200 Flow Cytometry (Fixed/Permeabilized): 1:800 -1:3200 Note: Anti-rabbit secondary antibodies must be used to detect N2303-01B Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice