N2301-40
NFkB Activating Kinase, CT (Nuclear Factor kappa B, TANK-Binding Kinase 1, NAK, TBK1)
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£655.00
- SKU:
- N2301-40
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Raised against a synthetic peptide (ERFGSLTMDGGLRNVD) corresponding to amino acids 712 to 727 of human NAK/TBK1 (1,2), which are identical to those of mouse homologue (1).
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
- Weitere Details:
- Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. Phosphorylation of I-kB by IkB kinase (IKK) complex leads to degradation of I-kB and activation of NF-kB. The IKK complex contains IKKa, IKKb, and IKKg. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-kB-activating kinase), and T2K (1-3). NAK/TBK1 activates IKKb through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-kB activation in response to growth factors (2). NAK/TBK1 functions upstream of NIK and the IKK complex (1,2). NAK/TBK1 is also critical in protecting embryonic liver from apoptosis (3). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml; Optimal dilutions to be determined by the researcher. Positive Control: MOLT4 whole cell lysate Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice