N2116-41C

Nogo-B (Foocen, KIAA0886, My043, Nbla00271, Nbla10545, Neurite Outgrowth Inhibitor, Neuroendocrine-specific Protein, NI220/250, NSP, NSP-CL, Reticulon-4, Reticulon-5, RTN4-A, RTN4-B1, RTN4-B2, RTN4-C, RTN-x, RTN-X, SP1507, SP1507)

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£748.00

SKU:: N2116-41C
Anwendung:: IHC-Fr
Reinheit:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to Met1-Ile183 (predicted) from Nogo-B expressed in NSO cells.
Klon:: 11n137
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Weitere Details:: Nogo-B, is a 55kD splice form of Nogo-A/Reticulon-4 and is primarily expressed in vascular endothelial cells. Nogo-A contains a large N-terminal cytoplasmic domain and a reticulon region that consists of two transmembrane segments with an intervening noncytoplasmic domain. Nogo-B lacks most of the N-terminal domain but retains the reticulon domain. It inhibits neointimal hyperplasia in the vasculature and is downregulated in atherosclerotic tissue and thoracic arterial aneurisms. Interaction of Nogo-B with NgBR promotes endothelial cell proliferation and migration. Within aa1-183, mouse Nogo-B shares 76-94aa sequence identity with human and rat Nogo-B, respectively. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 8-25ug/ml using immersion fixed frozen sections of adult mouse heart Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice