NES (S1409) (Nestin, Nbla00170)

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£696.00

SKU:: N2060-04L
Reinheit:: Purified by Protein A and peptide affinity chromatography
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1389-1416 of human NES
Format:: Supplied as a liquid in PBS, 0.09% sodium azide
Weitere Details:: Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence, Indirect Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:2000 Flow Cytometry (Indirect): 1:25 Immunohistochemistry (FFPE): 1:25. Heat induced antigen retrieval with citrate buffer, pH 6.0 is recommended. Immunofluorescence: 1:25 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice