N2050-11P
CD271 (p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR, NGFR, Neurotrophin, Nerve Growth Factor Receptor, p75, TNR16) (FITC)
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£652.00
- SKU:
- N2050-11P
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by affinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- WM245 melanoma cells.
- Klon:
- ME20.4
- Format:
- Supplied as a liquid in PBS, pH 7.2, BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
- Weitere Details:
- CD271, also known as p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR, and NGFR, is a type I transmembrane protein with a MW of 75 kD. It is expressed by many cell types including neurons, Schwann cells, mesenchymal stem/stromal cells, follicular dendritic cells and melanocytes. The extracellular portion contains four TNFR-Cys repeats that form the binding domain for its ligands (NGF, BDNF, NTF3, and NTF4). The intracellular portion of CD271 contains a death domain, which interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9, to promote cell apoptosis, and to regulate cell differentiation and neurogenesis. Applications: Suitable for use in Flow Cytometry. Though not tested, according to references additional reported applications include: Immunoprecipitation (ref. 1), Western Blot (ref. 4), Immunohistochemistry staining (frozen or paraffin embedded tissue sections) (ref. 1 and 2) and Blocking of NGF binding (ref. 1). Other applications not tested. Recommended Dilution: Flow Cytometry: 5ul/10e6 cells in 100ul Optimal dilution determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice