N2050-06K
Nerve Growth Factor, beta (NGF, Beta Nerve Growth Factor, Beta Nerve Growth Factor Precursor, Beta NGF, HSAN5, Nerve Growth Factor beta, Nerve Growth Factor beta Polypeptide, Nerve Growth Factor beta Subunit, NGFB)
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£735.00
- SKU:
- N2050-06K
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE).
- Format:
- Supplied as a lyophilized powder in PBS, pH 7.4. Reconstitute with 500ul sterile-filtered PBS, pH 7.2-7.6. Centrifuge to remove any insoluble material.
- Weitere Details:
- Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. Applications: Suitable for use in Immunohistochemistry, ELISA, Western Blot and Inhibition of biological activity in vitro. Other applications not tested. Recommended Dilutions: Immunohistochemistry (frozen): 1-3ug/ml Western Blot: 1-3ug/ml ELISA: 1-3ug/ml Inhibition of biological activity in vitro: 1-3ug/ml Use neat for in vivo studies: 2-10ug/mL (ED50). Optimal dilution determined by the researcher. Biological Activity: Whole sheep antisera will completely inhibit the survival and neurite outgrowth actions of mouse NGF in chicken dorsal root ganglion neurons in vitro. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice