N1193-10M
Netrin G1 Ligand Precursor (NGL-1, LRRC4C, leucine rich repeat containing 4C KIAA1580, UNQ292, PRO331) (Biotin)
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£908.00
- SKU:
- N1193-10M
- Anwendung:
- WB
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Purified, NS0-derived, recombinant human NGL-1 extracellular domain (rhNGL-1)
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
- Weitere Details:
- The Netrin-G1 ligand (NGL-1) promotes the outgrowth of thalamocortical axons. Human netrin G1 ligand is a transmembrane protein containing leucine-rich repeat (LRR) and immunoglobulin (Ig) domains that specifically interacts with netrin-G1 through its LRR region. Postsynaptic netrin-G ligand (NGL) CAMs associate with netrin-G CAMs in an isoform-specific manner and, through their cytosolic tail, with the abundant postsynaptic scaffold postsynaptic density-95 (PSD-95). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Westerm Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect human NGL-1. The detection limit for rhNGL-1 is approximately 2ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice