NALP3 (NACHT-, LRR- and PYD-Containing Protein 3, AGTAVPRL, AII/AVP, Angiotensin/Vasopressin Receptor AII/AVP-like, C1orf7, Cold Autoinflammatory Syndrome 1 Protein, CIAS1, Cryopyrin, Familial Cold Autoinflammatory Syndrome, FCAS, FCU, Muckle-Wells Syndrome, MWS, PYRIN Containing APAF1-like Protein 1, PYPAF1)

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£876.00

SKU:: N0018-64J1
Anwendung:: WB
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to aa4-223 from human NALP3 expressed in E. coli (Q96P20).
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Weitere Details:: NALP3 is a cytoplasmic 115-130kD member of the NLRP family of molecules. It is selectively expressed, being found in stratified squamous and transitional epithelium, Hassell's corpuscles, neutrophils, dendritic cells and T cells. NALP3 promotes IL-1b and IL-18 maturation by activating caspase-1. It does so by forming a NALP3 inflammasome comprised of NALP3, ASC and caspase-1. Bacterial RNA and cell wall peptidoglycan can bind to NALP3, promoting (potentially) ATP binding, NALP3 oligomerization, and caspase activation. Human NALP3 is 1036aa in length. It contains an N-terminal DAPIN domain (aa1-93), a NACHT domain (aa220-536), and seven consecutive LRRs (aa740-991). Alternate splice forms are reported. Either individually, or in combination, there can be deletions of aa836-892, 721-777, and a premature truncation after Gly719 that generates a 70kD isoform. Over aa3-223, human NALP3 shares 76aa identity with mouse NALP3. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice