M9601-30C
Myc-Tag (c-myc, myc-1 epitope tag) (HRP)
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£895.00
- SKU:
- M9601-30C
- Anwendung:
- WB
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to aa410-420 (MEQKLISEEDL) of human Myc (KLH).
- Klon:
- 4H357 (4A6)
- Format:
- Supplied as a liquid in PBS, 0.2mg/ml BSA, 0.01% thimerosal, 50% glycerol. Labeled with horseradish peroxidase (HRP).
- Weitere Details:
- Myc-Tag is a short synthetic peptide sequence derived from C-terminal region of human c-myc that is used as an epitope tag. The Myc tag can be cloned either upstream or downstream of the target gene, resulting in a fusion protein that allows efficient purification, detection, and localization of target proteins. The relatively small size of this tag vs. other commonly used tags such as GST (30kD) makes it less likely to interfere with target protein structure or function. The myc epitope tag is widely used to detect expression of recombinant proteins in bacterial, yeast, insect, and mammalian systems. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500 detects Myc-PP2A on 10ug of Myc-PP2A A subunit transfected NIH/3T3 lysates which was resolved by electrophoresis, transferred to PVDF membrane and probed with M9601-30C. Proteins were visualized using a donkey anti-rabbit IgG (HRP)* and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher. *Recommended Secondary Antibodies: I1904-46E: IgG, H&L (HRP) DkxRb I1904-46K: IgG, H&L X-Absorbed (HRP) DkxRb Positive Control: Widespread expression including A431 cell lysate, ovarian cancer cell lysate or breast carcinoma tissue. Myc fusion protein expressed in cells. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice