M9531

MUSK, Recombinant, Human, His-Tag, aa530-869 (Muscle/Skeletal Receptor Tyrosine Protein Kinase)

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£912.00

SKU:: M9531
Molekulargewicht:: 38.5
Reinheit:: Purified ≥60%
Lagerbedingungen:: -70°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 100mM sodium chloride, 0.05% tween 20, 3mM DTT, 20% glycerol.
Weitere Details:: Source: Recombinant corresponding to aa530-end of human MUSK, fused with N-terminal His-Tag, expressed in Baculovirus infected Sf9 cell expression system (NM_005592). Specific activity: 6.9pmole/min/ug. Assay conditions: Add 50ul reaction mix (MUSK diluted in 1x kinase buffer supplemented with 0.2mM ATP) to wells coated with substrate. Incubate for 1 hour. Add Europeum-labeled anti p-Tyr antibody and incubate for 1 hour. Time resolved fluorescence measured at RT at lambda-exc=330±40nm and lambda-em=620±10nm. Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 12 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.