M4679-50A
MP1 (MEK partner 1)
Größe
£780.00
- SKU:
- M4679-50A
- Reinheit:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- E. coli-derived recombinant protein corresponding to Ala2-Ser124 from human MP1.
- Klon:
- 7H255
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Weitere Details:
- MEK Partner 1 (MP1) was initially identified as a scaffold protein that tethers MEK1 and ERK1 to facilitate their activation. MP1 and other MAPK scaffolds promote phosphorylation efficiency and specificity, localize their bound pathway components to particular subcellular sites, and serve as nodes of signal integration for regulation of MAPK pathways by other signaling events. With the assistance of adaptor protein p14, the bound MP1 complex localizes to late endosomes and is thought to regulate endosomal traffic and cellular proliferation during tissue homeostasis. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml with HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, Balb/3T3 mouse embryonic fibroblast cell line, NRK rat normal kidney cells, and PC-12 rat adrenal pheochromocytoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice