M3884-91C
MGMT, Recombinant, Human (O6-Methylguanine DNA Methyltransferase)
Größe
£353.00
- SKU:
- M3884-91C
- Anwendung:
- WB
- Molekulargewicht:
- 23.8
- Reinheit:
- Purified ≥ 95% by SDS-PAGE
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Format:
- Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.5, 1mM DTT, 10% glycerol. Endotoxin: ≤1.0 EU/ug of protein (LAL)
- Weitere Details:
- O-6-methylguanine-DNA methyltransferase (MGMT) is an enzyme that repairs O-6-methylguanine, a mutagenic DNA base damaged by endogenous and environmental alkylating agents and is involved in the cellular defense against the biological effects of O-6-methylguanine in DNA. MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. There are few reports that abnormal MGMT expression correlates with the prognosis in human solid cancers. Recombinant MGMT, fused to His-tag at N-terminus, was expressed in E.coli and was purified by conventional chromatography techniques. Source: Recombinant corresponding to aa1-207 of human MGMT (His-tagged) expressed in E.coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYF HQPEAIEEFP VPAFHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 3ug/ml 15% SDS-PAGE, ~23.8 kD. Optimal dilutions to be determined by the researcher. Endotoxin: ≤1.0 EU/ug of protein (LAL) Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice