M3016-01
Methionine Aminopeptidase 2 (MetAP-2)
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£863.00
- SKU:
- M3016-01
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Sf 21-derived, recombinant protein corresponding to Ala2-Tyr478 from human Methionine Aminopeptidase 2.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
- Weitere Details:
- In vitro, the metalloprotease MetAP-2 catalyzes the hydrolysis of N-terminal methionine residues. MetAP-2 is highly conserved across species. The catalytic domains of S. cerevisiae and human MetAP-2 proteins are ~80% homologous. The function of MetAP-2 in vivo remains to be determined. MetAP-2 has been identified, purified and cloned from rat cells as a 67kD eIF-2-associated (elongation inhibitory factor-2) protein, suggesting a possible role in protein translation. Provocative experiments suggest MetAP-2 may also be involved in angiogenesis, the process of new blood vessel formation. In these studies, MetAP-2 was determined to be the major cellular protein covalently bound to the potent anti-angiogenic agent fumagillin, following fumagillin treatment of HUVEC (human umbilical endothelial cells) cells or calf brain homogenates. Fumagillin was able to selectively inhibit the growth of a MetAP-2-dependent yeast strain. The fungal derived fumagillin, and its more potent metabolite TNP-470 (now in clinical trials), are effective at limiting both the size and metastasis of solid tumors. TNP-470 inhibits neovascularization via endothelial cell cycle arrest in the late G1 phase. The details of this process are not well understood. In view of the identification of MetAP-2 as a fumagillin binding protein, future studies with MetAP-2 should provide important new insights into the mechanisms of fumagillin/TNP- 470 induced cell-cycle arrest and the role of MetAP-2 in angiogenesis. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice