M3007-13
c-Met, CT (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)
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£856.00
- SKU:
- M3007-13
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 13aa located within last 50aa derived from the C-terminus of the human c-Met protein.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Available without azide, BSA and glycerol. See M3007-13A.
- Weitere Details:
- Following its characterization as an activated oncogene (based on its ability to transform normal fibroblasts) Met was characterized as the receptor for hepatocyte growth factor. The c-Met receptor is a disulfide bonded heterodimer consisting of an ~50kD extracellular a-chain and an ~145kD b-chain with a cytoplasmic tyrosine kinase domain. Both chains are derived from a single precursor protein by proteolytic cleavage. c-Met is expressed in a wide variety of adult and embryonic tissues. It transmits biological responses including mitogenesis, morphogenesis and anti-apoptotic activity elicited by HGF. Dysregulated c-Met gene expression is observed in a variety of human carcinomas and sarcomas. c-Met also mediates the movement and invasiveness of neoplastic cells and promotes metastasis. Recently, transcription of the c-Met gene was suggested to be a target for regulation by p53. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): 1:10-1:100 Requires HIER using sodium citrate buffer pH6.0 followed by microwaving for 8-15min prior to staining. Flow Cytometry: 1:100-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice