M2865-10B

MEK1, MEK2, phosphorylated (Ser218/222, Ser222/226) (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1 / MAPK/ERK Kinase 2, Dual Specificity Mitogen-activated Protein Kinase Kinase 2, MAP Kinase Kinase 2, MAP2K2, MAPKK 2, MKK2, PRKMK2, ERK Activator Kinase 2)

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£800.00

SKU:: M2865-10B
Anwendung:: WB
Reinheit:: Purified by Protein A affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Peptide containing DpSMANpSF in which pS corresponds to phosphorylated amino acids 218 and 222 of human MEK1
Klon:: 4H353
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Weitere Details:: The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:2000-1:10,000. Detects phosphorylated MEK1/2 in RIPA lysates from PC-12 cells that had been treated with 5u of B-Raf for 30 minutes at 30°C. Untreated and B-Raf phosphorylated PC-12 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with M2865-10B (1:5000) or Pab Rb anti-Mouse MEK1, NT (Map Kinase Kinase) (M2865-07) (1:1000). Proteins were visualized using a goat anti-rabbit secondary IgG labeled with HRP and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice