M2825-14B
MDR1 (Multi Drug Resistance Protein 1, ABC20, ATP Binding Cassette Subfamily B (MDR/TAP) Member 1, ABCB1, CD243, CLCS, GP170, P-Glycoprotein, P-gp, P Glycoprotein 1, PGY1)
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£863.00
- SKU:
- M2825-14B
- Reinheit:
- Purified by Protein G affinity chromatography from ascites.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human and hamster drug-resistant viable cells followed by crude plasma membranes. Cellular Localization: Cell membrane. Epitope: Extracellular amino half
- Klon:
- 0.N.505
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
- Weitere Details:
- Shows no crossreactivity with the human MDR3 product. Its epitope localizes in the extracellular amino terminal half of P-glycoprotein (at the third extracellular loop of the molecule). This epitope is resistant to formalin fixation and periodate oxidation1 and is different from those of other commonly known Mabs to P-glycoprotein, such as C219, JSB-1, and MRK16, which recognize an intracellular and extracellular epitope of P-glycoprotein, respectively. Detects soluble P-glycoprotein in the cultured media of viable adriamycin-resistant cells, whereas those of the drug-sensitive parent cells contain no detectable level of soluble P-glycoprotein. Soluble P-glycoprotein is also detected in extracellular fluids of cancer patients, such as malignant ascites and serum. The Mr of soluble P-glycoprotein is the same as that of membrane bound P-glycoprotein. Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: Fixed cells only. Western Blot: 1-2ug/ml for 2hrs at RT Immunohistochemistry: 2-4ug, for 30 minutes at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: Drug resistant cells, Kidney or breast carcinoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice