M2825-13C
MDR Protein (PE) (MRP1, Multidrug resistance-associated protein 1, ABCC1, ATP-binding cassette sub-family C member 1, Leukotriene C(4) transporter)
Größe
£1,004.00
- SKU:
- M2825-13C
- Reinheit:
- Purified
- Lagerbedingungen:
- 4°C Do not freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Non-denatured membranes prepared from the human small cell lung cancer cell line, H69AR, which highly overexpresses MRP1.
- Klon:
- 3H406 (QCRL-1)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
- Weitere Details:
- MRP1 is a 190kD transmembrane phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines and tumors (1-5). Applications: Suitable for use in Western Blot, Immunoprecipitation, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:100-1:500 (Anti mouse IgG labeled with HRP). Immunocytochemistry: 1:20-1:50. [PBS, 0.5% paraformaldehyde-fixed or 70% methanol-fixed cytospin preparations (2)]. Immunohistochemistry: FFPE sections following antigen retrieval by microwave heating in citrate (5). Flow Cytometry: Cells fixed in PBS, 0.5% paraformaldehyde for 30 minutes, followed by anti-mouse IgG labeled with FITC. Note: Block and wash with solution containing 0.1% Tween. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice