M2749-35-HRP
MCM5 (Mini Chromosome Maintenance Protein 5, CDC46, CDC46 Homolog, Cell Division Cycle 46, DNA Replication Licensing Factor MCM5, MGC5315, Minichromosome Maintenance Deficient (S. cerevisiae) 5, Minichromosome Maintenance Deficient Protein 5, P1-CDC46) (HRP)
Größe
£852.00
- SKU:
- M2749-35-HRP
- Reinheit:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Bacterially expressed human Mcm5.
- Klon:
- 3H2646 (CRCT5.1)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
- Weitere Details:
- MCM-5 (mini chromosome maintenance protein 5), a protein with an important role in the control of DNA replication. It has been reported that immunocytochemical assessment of Mcm5 expression may be of value in improving the accuracy of cervical smear testing for the detection of malignancy. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended. Optimal dilutions to be determined by the researcher. Recommended Positive Control Tissue: Tonsil/Cervical Carcinoma Hybridoma: Spleen cells from immunized CBI/CFI mice were fused with cells of the mouse SP2 myeloma cell line. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice