M2749-30-BIOTIN
MCM2 (Mini Chromosome Maintenance Protein 2, BM28) (Biotin)
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£852.00
- SKU:
- M2749-30-BIOTIN
- Reinheit:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Bacterially expressed human Mcm2.
- Klon:
- 3H2645 (CRCT2.1 (D1.9H5))
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Weitere Details:
- MCM2 (Mini chromosome maintenance protein-2), is a protein involved in the control of DNA replication. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilution: Immunohistochemistry (frozen): 1:500-1:1000, Immunohistochemistry (paraffin): 1:200 Note: Requires antigen retrieval using heat treatment prior to staining of paraffin sections (0.01M cutrate buffer, pH 6.0). Optimal working dilutions to be determined by researcher. Recommended Secondary Antibodies: I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control Hybridoma: Sp2 myeloma cells with spleen cells from CBA/c F1 mice. Recommended Positive Control Tissue: Tonsil, any tissue with high proliferative index Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice