M2441

Matrix Metalloproteinase MT1 (MT1 MMP, Membrane Type 1, MMP-14)

Größe

£819.00

SKU:: M2441
Reinheit:: Purified
Lagerbedingungen:: 4°C Do Not Freeze
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Peptide corresponding to aa319-333, CDGNFDTVAMLRGEM in the hemopexin-like domain of human membrane Type1 matrix metalloproteinase human MT1-MMP.
Klon:: 2Q526
Format:: Supplied as a liquid in PBS, pH 7.0, 2% BSA (protease free).
Weitere Details:: Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 10ug/ml, membrane preparations; MD-MB-231 cells pretreated with 1-5ug/ml ConA for 24-48 hours for positive control. 60-65kD proteins are identified. When tested against recombinant MT1-MMP (lacking the TM domain) expressed in CHO cells the antibody reacts with a band of ~59kD. Immunohistochemistry: Frozen sections; traditional formalin fixation problematic. Paraffin-embedded tissue sections with PLP fixation required. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.