M2423-20
Matrix Metalloproteinase 7, Active, Recombinant, Human (MMP-7, Matrilysin, Pump1, Uterine Metalloproteinase)
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£964.00
- SKU:
- M2423-20
- Molekulargewicht:
- 19130
- Lagerbedingungen:
- -70°C
- Hersteller:
- United States Biological
- Format:
- Supplied as a liquid in 10mM HEPES, pH 7.4, 5mM calcium chloride, 0.15M sodium chloride.
- Weitere Details:
- H-MMP-7 is a member of the Matrix metalloproteinase family of endopeptidases, containing the canonical HExxHxxGxxH motif as a zinc binding active site. MMP-7 and MMP-26 (Matrilysin-2) are the only MMPs discovered that completely lack a hemopexin “regulatory domain” or hinge region. Like the other MMPs, MMP-7 is secreted as a zymogen, then activated. The 28kD form is reduced to 18kD by enzymatic cleavage after the conserved “cysteine switch.” MMP-7 is not constitutively produced. It is produced on demand in specific tissues. MMP-7 substrate specificity is broad, most closely resembling the activity of MMP-3 (Stromelysin-1). When MMP-7 production is stimulated, native MMP inhibitors (TIMPs) usually follow to quench them. Applications: Suitable for use in ELISA, Western Blot (control or standard) and SDS-PAGE Standards. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Molecular Weight: 19,130 calculated from amino acid sequence and 20,000 from SDS-PAGE, followed by staining with CBB R-250. Specific Activity: ~5000 U/mg protein Unit Definition: One unit of enzyme activity is defined as the quantity required to digest 1ug of Azocoll/min. at pH 7.5 and 37°C. Storage and Stability: MMP-7, when stripped of TIMPs, is unstable, and must stored carefully. To avoid repeated freeze thaw cycles, aliquot and store the enzyme at -70°C. Aliquots are stable for 6 months after receipt at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Dry Ice