M2421-23

Matrix Metalloproteinase 3 (MMP-3, Stromelysin-1, SL-1, Transin)

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£927.00

SKU:: M2421-23
Reinheit:: Purified by Protein A affinity chromatography from ascites.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: APMA (4-Aminophenylmercuric acetate) activated Human stromelysin-1
Klon:: 2Q535 (SL-1 IID4)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Weitere Details:: MMPs are frequently expressed in malignant neoplastic cells and play crucial roles in tumor invasion and metastasis. Tissue inhibitors of matrix metalloproteinases (TIMPs) control the activitiy of MMPS. The tissue distribution of three major MMPs has been defined in human mammary pathology: 1) collagenase (MMP-1) which degrades fibrillar interstitial collagens; 2) a 72kD gelatinase (MMP-2) which mainly degrades type IV collagen and denatured collagens; and 3) stromelysin (MMP-3) which has a wider range of action, degrading several matrix components including the core proteins of proteoglycans, laminin and non-helical regions of collagens. Applications: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Immunoprecipitation (Use Protein A): 2ug/ml Protein lysate. Western Blot: 0.5-1.0ug/ml for 2hrs at RT Immunohistochemistry (frozen & formalin-fixed): 10-20g/ml for 60 minute at RT. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues. Optimal dilutions to be determined by the researcher. Cellular Localization: Cytoplasmic Positive Control: Human endometrium cells, placenta, bladder, breast, ovarian carcinomas. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice