M2363
MAP Kinase p44/42, phosphorylated (Thr202/Tyr204) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Kinase 2, MAPK 2, ERK2, PRKM1, PRKM2 / Mitogen-activated Protein Kinase 3, MAP Kinase 3, MAPK 3, ERT2, Extracellular Signal-regulated Kinase 1, ERK-1, Insulin-stimulated MAP2 Kinase, MAP Kinase Isoform p44, p44-MAPK, Microtubule-associated Protein 2 Kinase, p44-ERK1, ERK1, PRKM3)
Größe
£825.00
- SKU:
- M2363
- Reinheit:
- Purified by thiophilic absorption followed by size exclusion chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- KLH-conjugated phosphopeptide containing the pTEpY motif of Erk1 and Erk2.
- Klon:
- 0.T.87 (12D4)
- Format:
- Supplied as a lyophilized powder in PBS, PEG, sucrose, 0.09% sodium azide. Reconstitute with 1ml ddH2O.
- Weitere Details:
- Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Western Blot: 0.1-1ug/ml detects pMAPK in RIPA lysates from EGF-stimulated human A431 carcinoma cells. Non-stimulated A431 and EGF-stimulated A431 cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-pMAPK (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: Use 1-10ug per immunoprecipitation. Optimal dilutions to be determined by the researcher. Positive Antigen Control: EGF-stimulated A431 lysate. Use 20ug per lane for minigels. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice