M2363-95G-BIOTIN
MAP Kinase Kinase 7 (Dual Specificity Mitogen-activated Protein Kinase Kinase 7, MAP2K7, MAPKK 7, MAPKK7, MKK7, PRKMK7, c-Jun N-terminal Kinase Kinase 2, JNK Kinase 2, JNKK 2, JNKK2, JNK-activating Kinase 2, MAPK/ERK Kinase 7, MEK 7, MEK7, Stress-activated Protein Kinase Kinase 4, SAPK Kinase 4, SAPKK4, SAPKK-4, SKK4) (Biotin)
Größe
£914.00
- SKU:
- M2363-95G-BIOTIN
- Reinheit:
- Purified by Protein A chromatography
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant GST-fusion protein containing full length human MKK7 sequence.
- Klon:
- 2E3G1
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
- Weitere Details:
- Mitogen-activated protein kinase (MAP kinase) signal transduction pathways have been implicated in multiple physiological and pathophysiological processes including growth differentiation, survival and death. The p38 group of MAP kinases is activated by treatment of cells with proinflammatory cytokines and by exposure to environmental stress. One important function of the p38 signaling pathways appears to be the regulation of cytokine expression. The MAP kinases are activated by conserved protein kinase signaling modules, which include a MAP kinase kinase kinase (MKKK) and a dual-specificity MAP kinase kinase (MKK). The MKKK phosphorylates and activates the MKK, which, in turn, activates the MAP kinase by dual phosphorylation on threonine and tyrosine residues within a Thr-Xaa-Tyr motif located in protein kinase subdomain VIII. MKK7 is a novel activator of c-Jun N-terminal kinase (JNK). This 47-Kda protein is widely expressed in human and mice. The kinase domain of MKK7 is closely related to a Drosophila JNK kinase dHep (69% homology) and C. elegans (54% homology), and it is mildly related to MKK4, MKK3, and MKK6. MKK7 phosphorylates and activates JNK1 in vitro. Applications: Suitable for use in Immunofluorescence, Western Blot and ELISA. Other applications have not been tested. Recommended Dilution: Immunofluorescence: 15-20ug/ml Western Blot: 1-3ug/ml ELISA: 0.1-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice