M2363-95D
MAP Kinase Kinase 7, phosphorylated (Thr275/Ser277) (Dual Specificity Mitogen-activated Protein Kinase Kinase 7, MAP2K7, MAPKK 7, MAPKK7, MKK7, PRKMK7, c-Jun N-terminal Kinase Kinase 2, JNK Kinase 2, JNKK 2, JNKK2, JNK-activating Kinase 2, MAPK/ERK Kinase 7, MEK 7, MEK7, Stress-activated Protein Kinase Kinase 4, SAPK Kinase 4, SAPKK4, SAPKK-4, SKK4)
Größe
£767.00
- SKU:
- M2363-95D
- Anwendung:
- WB
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Peptide corresponding to amino acids surrounding Thr275/Ser277 of human MKK7/SKK4.
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
- Weitere Details:
- Dual specificity mitogen-activated protein kinase kinase 7 (UniProt: O14733; also known as EC:2.7.12.2, MAP kinase kinase 7, MAPKK 7, JNK-activating kinase 2, MAPK/ERK kinase 7, MEK 7;Stress-activated protein kinase kinase 4, SKK4, SAPK kinase 4, SAPKK-4, SAPKK4, c-Jun N-terminal kinase kinase 2,JNK kinase 2, JNKK 2) is encoded by the MAP2K7 (also known as JNKK2, MEK7, MKK7, RKMK7, SKK4) gene (Gene ID: 5609) in human. MKK4/SKK4 belongs to the STE Ser/Thr protein kinase family and is an essential component of the MAP kinase signal transduction pathway. MKK4/SKK4 is ubiquitously expressed with highest level of expression reported in skeletal muscle. It contains a single protein kinase domain and has a DVD domain (aa 377-400) that contains a conserved docking site and bind to their specific upstream MAP kinase kinase kinases (MAPK3Ks). MKK4/SKK4 also has a D domain in the N-terminal region (aa 37-57), which contains a conserved docking site that is required for the binding of MAPK substrates. It is activated by phosphorylation on Ser271 and Thr275 by MAP kinase kinase kinases (MAP3Ks) and it directly phosphorylates and activates the c-Jun NH2-terminal kinases (JNK), in response to cellular stresses and pro-inflammatory cytokines. Mice lacking the MKK4 gene are shown to have abnormal hepatogenesis and die before embryonic day 14. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500 detected Phospho-MKK7/SKK4 (Thr275/Ser277) in lysate from 10ug of A431 cells treated with Calyculin A and okadaic acid. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice