M2355-05

MAPKAP Kinase 2, Active, Recombinant, Human (Mitogen-activated Protein Kinase-activated Protein Kinase 2, MAP Kinase-activated Protein Kinase 2, MAPK-activated Protein Kinase 2, MAPKAPK2, MAPKAPK-2, MK2)

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£1,119.00

SKU:: M2355-05
Molekulargewicht:: 70.2
Reinheit:: ≥90%
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.1mM EGTA, 0.1% 2- mercaptoethanol, 0.02% Brij-35, 50% glycerol.
Weitere Details:: Recombinant human MAPKAP Kinase 2, residues 46-400, A399G, containing an N-terminal GST tag; expressed in E. coli. Specific Activity: As Reported Unit Definition: 1 unit of MAPKAP kinase 2 activity is defined as 1nmol phosphate incorporated into 30uM substrate peptide (KKLNRTLSVA), (Cat #M2355-09) per minute at 30°C with a final ATP concentration of 100uM. Kinase Assay: 2.2-6ng of enzyme phosphorylated 30uM MAPKAP kinase 2 substrate peptide MS Tryptic Fingerprint: Confirmed identity as MAPKAP kinase 2 with 23% amino acid coverage of the translated native sequence (see protocol). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.