M1202-78
Macrophage (L1 Protein, Calprotectin, Cystic Fibrosis Antigen) (FITC)
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£620.00
- SKU:
- M1202-78
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human monocytes
- Klon:
- 3H2617 (MAC387)
- Format:
- Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
- Weitere Details:
- L1 Protein or Calprotectin Molecule is an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and by tissue macrophages. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Neat. Use 10ul to label 10e6 cells in 100ul. Membrane permeabilization is required. Optimal working dilutions to be determined by researcher. Recommended Negative Control: I1904-78R3: IgG1 Murine Negative Control (FITC) Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice