M1202-76-HRP
Macrophage (L1 Protein, Calprotectin, Cystic Fibrosis Antigen) (HRP)
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£837.00
- SKU:
- M1202-76-HRP
- Anwendung:
- IHC-Fr
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human monocytes
- Klon:
- 3H2617 (MAC387)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
- Weitere Details:
- The L1 or Calprotectin molecule is an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen and Paraffin): Prior to staining paraffin sections, product requires protein digestion pre-treatment e.g. trypsin, 0.1% for 10 minutes or antigen retrieval using heat treatment. Frozen sections do not require pretreatment. Optimal working dilutions to be determined by researcher. Recommended Positive Control: Human spleen Recommended Negative Control: I1904-78R: IgG1: Murine Negative Control Recommended Secondary Antibodies: I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb x Mo I1904-66C: IgG, F(ab’)2 X-adsorbed (HRP) Pab Rb x Mo I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb x Mo Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice