M1202-65K

Macrophage Inflammatory Protein 3 beta (MIP-3-beta, MIP3B, MIP-3b, Beta Chemokine Exodus-3, C-C Motif Chemokine 19, Chemokine (C-C Motif) Ligand 19, CCL19, CK beta-11, CKb11, Epstein-Barr Virus-induced Molecule 1 Ligand Chemokine, EBI1 Ligand Chemokine, ELC, MGC34433, Small-inducible Cytokine A19, SCYA19) (Biotin)

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£657.00

SKU:: M1202-65K
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human MIP-3 beta, expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS. No preservative added. Labeled with Biotin. Reconstitute with 500ul sterile PBS, 0.1% BSA.
Weitere Details:: MIP-3 beta acts as a chemoattractant for T and B lymphocytes, dendritic cells, NK cells and myeloid progenitor cells, through specific binding to CCR7, a G-protein coupled receptor known to be up-regulated by Epstein-Barr virus infected B cells and Herpes virus infected T cells. A correlation between the response to MIP-3 beta and the level of CCR7 expression and has been revealed in the maturation of dendritic cells (DCs), and increased levels of both MIP-3 beta and CCR7 expression have been shown in bronchoalveolar lavage fluid (BALF) and BALF cells from patients with the granulomatous disorder sarcoidosis, associated predominantly with CD4+ T lymphocyte alveolitis, and hence T cell recruitment. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA (sandwich): 0.25-1ug/ml. (100ul/well) in conjunction with M1202-66 as a capture antibody, allows the detection of at least 0.2- 0.4ng/well of recombinant human MIP-3β. Western Blot: 0.1-0.2ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3β is 1.5-3ug/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice