L9375-01A
LYVE-1 (Lymphatic Vessel Endothelial Hyaluronic Acid Receptor, LYVE1, CRSBP1, Extracellular Link Domain Containing 1, Hyaluronic Acid Receptor, HAR, Lymphatic Endothelium Specific Hyaluronan Receptor, Lymphatic Vessel Endothelial Hyaluronan Receptor 1, XLKD1)
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£1,029.00
- SKU:
- L9375-01A
- Reinheit:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant. Endotoxin: ≤0.1EU/ug (LAL)
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Ser24-Thr238 of human LYVE-1, exprssed in NS0 cells.
- Klon:
- 9j8
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1ml sterile PBS.
- Weitere Details:
- LYVE-1, also named extracellular link domain containing 1, is a 60-70kD type I transmembrane protein that functions as a receptor for both soluble and immobilized hyaluronan (HA). It shares 41% aa sequence homology with CD44, another known receptor for HA. LYVE-1 expression is mostly restricted to lymphatic vessels. LYVE-1 is a useful molecular marker for lymphatic endothelial cells. Applications: Suitable for use in Direct ELISA and Blocking of receptor-ligand interaction. Other applications not tested. Recommended Dilutions: Blocking of receptor-ligand interaction: 0.04-0.2ug/ml will block 50% binding of 1.5ug/ml of biotinylated Hyaluronan to immobilized rhLYVE-1 coated at 5ug/ml. At 2ug/ml, it will block >90%. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice