L8015-03E
Lymphotoxin beta Receptor (LTBR, LT-beta-R, Tumor Necrosis Factor C Receptor, Tumor Necrosis Factor Receptor Superfamily Member 3) (Biotin)
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£639.00
- SKU:
- L8015-03E
- Reinheit:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant human Lymphotoxin beta Receptor
- Klon:
- ANCLTR2/9E2
- Format:
- Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
- Weitere Details:
- Human Lymphotoxin beta receptor (LTbR, TNFSF3) is a member of the TNF receptor superfamily with similarilty to CD120a(TNFR1) and CD120b(TNFR2). It is a expressed mainly on non lymphoid tissues(1) and appears to have an important role in secondary lymphoid organ development(5). Suppression of LTbR signaling can alleviate autoimmunity(2) or exasperate mycobacterial infection(6). Its ligands include Ltab2 and LIGHT. In mice, LTbR signaling is important for development and function of HEV(2), Dendritic cells(3), and Mast cells(4). Applications: Suitable for use in Flow Cytometry and ELISA. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ug/ml; 80ul labels 5x10e5 cultured human U-937 cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 human U-937 cells per tube were washed and pre-incubated 5 minutes with 20ul 250ug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80ul of L8015-03E at 10ug/ml. 2.Cells were washed twice and incubated with secodary reagent Streptavidin/R-PE after which they were washed three times, fixed and analyzed by FACS. 3.Cells stained positive with a mean shift of 0.64 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. 4.Binding was blocked when cells were pre blocked with 20ul of 0.5mg/ml unlabeled anti-Lymphotoxin beta Receptor antibody Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice