L7732-01C

Ly49C,F,H,I (PE)

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£350.00

SKU:: L7732-01C
Anwendung:: Flow Cytometry
Reinheit:: Purified
Lagerbedingungen:: 4°C Do Not Freeze
Hersteller:: United States Biological
Host:: Hamster
Klon:: 14B11
Format:: Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE.
Weitere Details:: Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated.2 These inhibitory receptors are also expressed on a subpopulation of mouse CD8+ T cells. The antibody stains ~ 90% of NK1.1+CD3- splenocytes, and ~ 6% of CD8+ spleen cells from C57BL/6 mice.1 The antibody also stains 30-80% of NK1.1+CD3- splenocytes in other mouse strains tested (BALB/c, C3H, 129, SJL, AKR/J). Applications: • Flow cytometry 1 Recommended Dilution: Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.