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L7715-34

Ly-6C (Ly6C, Lymphocyte Antigen 6C2, Ly6c2, Ly-6C2, AA682074, AA959465)

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£626.00

SKU:
L7715-34
Reinheit:
Purified by Protein G affinity chromatography from tissue culture supernatant
Lagerbedingungen:
-20°C
Hersteller:
United States Biological
Host:
Rat
Immunogen:
Balb/c macrophage precursor cell hybrids
Klon:
ER-MP20
Format:
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Weitere Details:
Ly-6C is a 14kD differentiation antigen, which is expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and and T-lymphocytes. Clone ER-MP20 is able to distinguish multiple mouse blood monocyte subsets: immature Ly-6Chi monocytes are recruited to acute peripheral inflammation and develop into Ly-6C+ exudate macrophages, whereas more mature Ly-6C-/lo monocytes are precursors for tissue macrophages and dendritic cells in steady state. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: Y3-Ag1.2.3 rat myeloma cells with spleen cells from immunized rats. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Versandbedingungen:
Blue Ice