L7715-05A
Ly-6A/E (Ly6A/E, Lymphocyte Antigen 6A-2/6E-1, Ly-6A.2/Ly-6E.1, Ly6a, Ly6, Ly-6A.2, Ly-6E.1, Stem Cell Antigen 1, Sca1, SCA-1, T-cell-activating Protein, TAP, Lymphocyte Activation Protein 6) (FITC)
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£499.00
- SKU:
- L7715-05A
- Reinheit:
- Purified
- Lagerbedingungen:
- 4°C (-20°C Glycerol)
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- IL-2-dependent mouse T-cell line CTL-L
- Klon:
- D7
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with FITC.
- Weitere Details:
- Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E+ lymphocytes. The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation. Studies with this antibody suggest that Ly-6A/E may be involved in B and T lymphocyte responses and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation. Applications: Suitable for use in Flow cytometry, Immunoprecipitation, Western Blot, Immunohistochemistry and In vitro lymphocyte activation. Other applications have not been tested. Recommended Dilution: Flow Cytometry: ≤ 1ug/10e6 cells Immunohistochemistry: frozen sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
- Versandbedingungen:
- Blue Ice