L2601-04C
LRP4 (Low Density Lipoprotein Receptor-related Protein 4, KIAA0816, LRP10, LRP-4, MEGF7, Multiple Epidermal Growth Factor-like Domains 7)
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£856.00
- SKU:
- L2601-04C
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to Gly18-Ser344 from human LRP-4, expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Weitere Details:
- LRP-4 (Low density lipoprotein-related protein 4; also MEGF7 and LRP13) is a 215-225kD member of the LDLR family of proteins. It is expressed on neurons, oocytes, spermatogonia and skeletal muscle cells, and binds multiple ligands, including WISE, apoE, MuSK and neuronal Agrin. It serves to negatively regulate Wnt signaling during development, and to cluster AChRs at neuromuscular junctions. Mature human LRP-4 is an 1885aa type I transmembrane glycoprotein. It contains a 1705aa extracellular domain (ECD) (aa21-1725) plus a 159aa cytoplasmic region (aa1747-1905). In the ECD, there are eight LDLR class A repeats (aa26-350), two EGF-like repeats (aa354-434) and 20 LDLR class B repeats that contain an intervening EGF-like domain (aa480-1610). Over aa18-344, human LRP-4 shares 88% aa identity with mouse LRP-4. Applications: Suitable for use in Direct ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml in human brain (cortex) tissue and rat cortical embryonic neuron/glial cells Immunohistochemistry: 5-15ug/ml in immersion fixed paraffin-embedded sections of human brainstem Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice