L2499-15P
Lipocalin 2 (Lipocalin-2, Lcn2, 24p3, 25kD alpha-2-microglobulin-related Subunit of MMP-9, HNL, MSFI, Neutrophil Gelatinase-associated Lipocalin, NGAL, Oncogene 24p3, p25) (Biotin)
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£953.00
- SKU:
- L2499-15P
- Reinheit:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Gln21-Asn200 from mouse Lipocalin-2, expressed in NS0 cells.
- Klon:
- 11n126
- Format:
- Supplied as a lyophilized powder in PBS, BSA. Reconstitute with sterile TBS, pH7.3 (20mM Trizma base, 150mM sodium chloride), 0.1% BSA to 500ug/ml. Labeled with Biotin.
- Weitere Details:
- Mouse Lipocalin-2 was cloned from mouse kidney cells (1). Its very high level of expression at the post-stratum uterus gave it the name uterocalin (2). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3-5). Studies indicate that Lipocalin-2 binds a bacterial catecholate siderophore that is bound to a ferric ion, such as enterobactin, with a subnanomolar dissociation constant (KD = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less KD values (7.9 nM) (6). Secretion of Lipocalin-2 in immune cells increases in response to stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing the epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8). Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA (detection): 2-8ug/ml using L2499-15Q as capture antibody Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice