Leukocyte Immunoglobulin-Like Receptor C1 (LILRC1)

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£863.00

SKU:: L2028-15
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Goat
Immunogen:: NS0-derived recombinant rat LILRC1 (aa17-248)
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Weitere Details:: Leukocyte Immunoglobulin-Like Receptor C1 (rrLILRC1) is a 36kD member of the LILR family. Mature rat LILRC1 is a 279 amino acid type I transmembrane protein with two extracellular lg-like domains and a cytoplasmic tail. The transmembrane region contains an Arg residue, suggesting an association with activating adaptor proteins. LILRC1 is found on B cells, neutrophils and macrophages. Over the region used as immunogen, rat LILRC1 protein is 84% identical to mouse LILRC1 and 58% identical to human LILRA5. Applications: Suitable for use in ELISA, Western Blot, Immunocytochemistry, Flow Cytometry Recommended Dilution: Direct ELISA: 0.5-1.0ug/ml with the appropriate secondary reagents to detect rat LILRC1. The detection limit for rrLILRC1 is approximately 1.5ng/well. In this format, this antibody shows approximately 30% cross-reactivity with rmLILRCI and less than 1% cross-reactivity with rrLILRC2. Optimal dilutions should be determined by each laboratory for each application. Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect rat LILRC1. The detection limit for rrLILRC1 is approximately 5ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 2-15ug/ml. This antibody will detect human rat LILRC1 in rat splenocytes. Flow Cytometry: Dilute to 50ug/ml and add 10ul of the diluted solution to 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as anti-goat lgG conjugated to a fluorochrome. This antibody has been tested on rat splenocytes for use in flow cytometry. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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