L1565-01X-ML650
Lck, p56 (Lymphocyte-specific Tyrosine Kinase) (BSA & Azide Free) (MaxLight 650)
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£1,183.00
- SKU:
- L1565-01X-ML650
- Reinheit:
- Purified by Protein A affinity chromatography from ascites.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa1-225 of mouse p56lck protein. Cellular Localization: Cell membrane
- Klon:
- 0.N.385
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Weitere Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000 p56lck is a membrane-associated non-receptor protein tyrosine kinase from the Src family of kinases. The phosphorylation status and the activity of p56lck is regulated by the CD45 tyrosine protein phosphatase. p56lck plays a critical role in T cell development and activation. p56lck gene is localized to a site in the genome which undergoes frequent chromosomal abnormalities in lymphomas and neuroblastomas. Applications: Suitable for use in ELISA, Immunoprecipitation, Kinase Assay, and Western Blot. Other applications have not been tested. Recommended Dilution: Immunoprecipitation: Use Protein A. 2ug antibody/mg protein lysate Western Blot: 1-2ug/ml for 2 hours at room temperature. Optimal dilutions to be determined by the researcher. Positive Control: Jurkat cells Cellular Localization: Cell membrane Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice