L1227-10X-ML650
Laminin B2, gamma1 (BSA & Azide Free) (MaxLight 650)
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£1,190.00
- SKU:
- L1227-10X-ML650
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- Murine EHS laminin preparation. Cellular Localization: Basement membrane.
- Klon:
- 0.N.384 (A5)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Weitere Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000 Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. They are ubiquitously present in basement membrane (BM) along with entactin/nidogen (EN), collagen type IV (CIV) and large heparan sulfate proteoglycan (HSPG). All interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. Applications: Suitable for use in Immunofluorescence, Immunoprecipitation, and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Immunoprecipitation (Native and denatured): 2ug/mg protein lysate using Protein G. Immunohistochemistry (acetone-fixed, frozen and acid-alcohol-fixed, paraffin-embedded): 2ug/ml for 30 min at RT. Note: Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 min at 37°C. Optimal dilutions to be determined by the researcher. Recommended Positive Control: LS174T cells, normal colon or colon carcinoma. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice