K1851-ML550
Kip2, p57 (cdk Inhibitor, Cyclin Dependent Kinase Inhibitor) (MaxLight 550)
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£1,023.00
- SKU:
- K1851-ML550
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa303-316 (C)GVGSVEQTPRKRLR of human Kip2 (KLH). Species sequence homology: mouse-14/15.
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
- Weitere Details:
- MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. The eukaryotic cell cycle is driven by the sequential action of a family of serine/threonine kinases, the cyclin dependent kinases (cdks) acting in concert with their regulatory subunits, the cyclins. p57 Kip2 is a potent inhibitor of several G1 cyclin/cdk2 complexes but unlike p27 Kip1 is not regulated by p53. Kip2 is thought to be involved in decisions to exit the cell cycle during development and differentiation. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml detects Kip2(~57kD) in a HeLa nuclear extract and a bovine brain nuclear extract. Immunoprecipitation: 5ug immunoprecipitates Kip2 from a HeLa nuclear extract and a bovine brain nuclear extract. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb Positive Antigen Control Included: H1838: HeLa Nuclear Extract Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice