K1000-07-ML650
Keratinocyte Growth Factor (KGF, Fibroblast Growth Factor 7, FGF-7) (MaxLight 650)
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£843.00
- SKU:
- K1000-07-ML650
- Reinheit:
- Purified by Immunoaffinity chromatography. Endotoxin: <0.1ng/ug protein
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human Keratinocyte Growth Factor expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Weitere Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Applications: Suitable for use in FLISA, Neutralization, and Western Blot. Other applications not tested. Recommended Dilution: FLISA (Indirect): 0.5-2ug/ml. Using 100ul/well antibody solution. When used in conjunction with the compatible secondary reagents, the detection limit is of 0.2-0.4ng/well. FLISA (Sandwich): 0.5-2ug/ml. Using 100ul/well antibody solution. When used in conjunction with K1000A, as a detection antibody, the detection limit is of 0.2-0.4ng/well. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: 0.5ug/ml, overnight at 4°C. This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. An HRP-labeled polymerdetection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Neutralization: 0.56-0.85ug/ml, to yeild one-half maximal inhibition [ND50] of the biological activity of hKGF (25ng/ml). Optimal dilution determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice