K0300-02D
Keyhole Limpet Hemocyanin (KLH)
Größe
£767.00
- SKU:
- K0300-02D
- Reinheit:
- Ascites
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- KLH protein from horseshoe crab.
- Klon:
- 11C129
- Format:
- Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
- Weitere Details:
- Small peptides, proteins or haptens (≤5kD) are not very antigenic when injected into animals. Antigenicity of haptens can be increased tremendously if they are covalently conjugated to bigger proteins referred as carrier proteins. Most common carrier proteins are albumin (BSA), thyroglobulin, ovalbumin, and hemocyanin from invertebrates. Hemocyanin are blood/lymph proteins found in only two groups: the mollusks and the arthropods. Keyhole limpet hemocyanin (KLH) is particularly preferred carrier proteins due to its very large size and antigenicity in common host species. Any carrier protein-hapten conjugate typically induces antibody responses to the epitopes on the carrier protein and the hapten. Antibody response can be followed by ELISA using either the carrier protein or the free hapten or free hapten conjugated to another carrier protein. Availability of antibodies to KLH may serve as positive control for the presence of anti-KLH antibodies. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:5000-1:20,000 Western Blot: 1:1000-1:10,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice