K0075-04F

KAP-1 (KRAB-Associated Protein 1, KAP1, FLJ29029, KRAB Interacting Protein 1, KRIP1, RING Finger Protein 96, RNF96, Transcription Intermediary Factor 1-beta, TIF1-beta, TF1B, TIF1B, Tripartite Motif-Containing 28, TRIM28, Nuclear Corepressor KAP-1)

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£429.00

SKU:: K0075-04F
Reinheit:: Purified by affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Klon:: 20A1
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Weitere Details:: TIFB (transcription intermediary factor 1-beta) is an 88kD member of the tripartite motif family. This protein contains three zinc binding domains, a RING domain, a B-box type 1 and type 2 domain, and a coiled-coil region. TIFB is found in the nucleus and associates with specific chromatin regions. This protein forms a complex with KRAB-domain transcription factors and recruits setdbl to histone 3 to increase KRAB-mediated transcriptional repression. TIFB has been reported to interact with setdbl and cbx3 proteins. This monoclonal antibody has been shown to recognize the human TIF1B protein and has been shown to be useful for Western blotting. Applications: Suitable for use in Western Blot and Immunocytochemistry. Not recommended for ChIP. Other applications not tested. Recommended Dilution: Western Blot: 0.025-0.1ug/ml Immunocytochemistry: 2.5-10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.